Short-interfering RNAs (siRNAs) have proved to be a useful tool in studying gene function in plants, invertebrates and mammalian systems. Herein, we report the use of siRNAs for targeting the human MAP kinase-interacting kinase Mnk1 gene. This study demonstrates the efficacy of the designed siRNA in silencing Mnk1 in the human cell line HEK293T and shows that Mnk1 suppression decreases eukaryotic initiation factor 4E Nike Air Max 1 Toronto
phosphorylation without causing any change in global protein synthesis rate and cell proliferation. Interestingly, suppression of Mnk1 results in a significant increase in eukaryotic initiation factor 4F complex formation after 72
h of transfection.
Trap behaviors in AlGaN–GaN heterostructures were characterized by the means of mercury-probe capacitance–voltage (C–V) technique for samples grown on sapphire and SiC substrates. The effective trap density was determined by a simplified model to give a schematic explanation for the capacitance dispersion. Fluorine plasma treatment Air Max 90 Em
was carried out with appropriate time and power for further identification. The VGa-related defects in (Al)GaN bulk layers were responsible for the frequency-dependent capacitance dispersion in pinch-off region, while the surface status influenced the capacitance dispersion in pinch-on region directly.